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Introduction:

The AX series are Nikon’s newer generation confocal laser scanning microscopes — engineered for high resolution, large field-of-view imaging, with faster speeds, better detectors, and modularity. The AX MP adds multiphoton capability to that, enabling deeper imaging in thick or living tissues (less scattering, less phototoxicity), plus super-resolution options with advanced detectors.

Applications:

• Very large field of view (FOV): up to 25 mm diagonal in confocal mode. That means you can image larger specimens (or larger portions of specimens) without stitching.
• High pixel resolution: up to 8K × 8K (8192 × 8192) for confocal imaging.
• High speed scanning: resonant scanning in the AX R / AX R MP allows very fast frame rates, especially for small ROIs. Example: ~720 fps at 2048 × 16 pixels.
• Improved detectors:

  - Lower noise, higher sensitivity detectors (GaAsP, PMTs, etc.) that enhances signal-to-noise, allows lower excitation power, and enables super-resolution type improvements.

• Multiphoton capability (in AX R MP): deep tissue imaging, less photodamage, ability to image in near-infrared (IR) spectrum, etc.

• Glass slides
• Chambered glass slides (Ibidi…)
• Circular glass/polymer/plastic bottom culture dishes (diameter: 20mm, 25mm, 30mm, 35mm,40mm). Be careful to check the working distance of the objective when using plastic bottom dishes
• * 4, 12, 24, 48,96 well plates with plastic/glass bottom. Be careful to check the working distance of the objective when using plastic bottom plates*

• 4X (Plan, Apo, 荡, NA 0.1 , WD 30mm)
• 10X (Plan, Apo, 荡, NA 0.45, WD 4mm)
• 20X (Plan, Apo, LWD, 荡S, Air, NA 0.95, WD 950um, water)
• 40X (Apo,  LWD, Water, DIC,NA 1.15, WD 610um)
• 60X (Plan, Apo LWD, 荡S, DIC, Water, NA 1.42, WD 150um)
• 100X (SR, Apo, TIRF, Oil, DIC, NA 1.49, WD 150mm)

• Confocal: LUA-S4 Laser Unit 405/ 488/ 561/ 640
• Multiphoton:  Coherent Chameleon Discovery
• Two channel: Tunable(820-1300nm) and Fixed (1040nm)
• Nikon D-LED white light source (for epifluorescence)
• LED lamp (for diascopic transmission light illumination)

• Galvano scanner (High quality image acquisition upto resolution 8192 X 8192 pixels)
• Resonant scanner (Fast image acquisition upto 30FPS at resolution 512 X 512 pixels)

• Descanned detectors for both Confocal and Multiphoton modes:
  • MultiAlkali(1):Fixed detection range(420-476nm)
  • GaAsP(3):One fixed detection range(662-737nm) and two tunable(400-750nm)
• NonDescanned Detectors (NDD) for multiphoton mode:
  • 4 fixed detection range detectors (415-485nm, 506-594nm, 604-676nm, 698-750nm)

• Fully motorized
• Can be programmed and setup for multipoint automated imaging experiments
• Nikon Perfect Focus System (PFS) for hardware based autofocus based on detection of the reflection from the coverslip
• Autofocus: based on contrast of the images from different in the z-stack
• Water dispenser for 60X Objective
• Option to add incubation chamber for temperature control and CO2 supply for live cell imaging